SUZ12 Depletion Suppresses the Proliferation of Gastric Cancer Cells

SUZ12 Depletion Suppresses the Proliferation of Gastric Cancer Cells

Blog Article

Background/Aims: SUZ12 and EZH2 are two main components of polycomb repressive complex 2 (PRC2) that is known to be of great importance in tumorigenesis.EZH2 has been reported to play a vital role in pathogenesis of human cancer.However, whether SUZ12 has equivalent roles in tumorigenesis has not been demonstrated.Here, we investigated a possible role of SUZ12 for the proliferation of gastric cancer cells.

Methods: Western-blot analysis was used to detected the levels of SUZ12, H3K27me3, EZH2 and p27 in ten gastric Pre-Rolled cell lines.SUZ12 was depleted by RNA interference.Cell cycle was Body detected by flow cytometry.Luciferase assays was to analyze whether miR-200b directly regulate SUZ12.

Results: We found that SUZ12 depletion mediated by RNA interference (RNAi) led to a reduction of gastric cell numbers and arrested the cell cycle at G1/S point.As an important G1/S phase inhibitory gene, p27 is re-induced to some extent by SUZ12 knockdown.Furthermore, we demonstrated that SUZ12 was directly downregulated by miR-200b.Conclusion: We provide evidence suggesting that SUZ12 may be a potential therapeutic target for gastric cancer.